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THERMAL CYCLERS

Thermal cyclers are used extensively in molecular biology as part of the PCR technique. To amplify a DNA fragment, the equipment is programmed to carry out several temperature cycles, generally up to 96 °C, so that the DNA in the sample is denatured, hybridised and expanded. Real-time thermal cyclers can quantify DNA at any time in the process based on a fluorescence signal, while conventional or end-point thermal cyclers are only qualitative and the results are only obtained at the end of the process after reading an agarose gel.

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